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RNA polymerase II

Elongation Forms and antibodies

Ab

polymerase

4H8

recognizes both the phosphorylated and unphosphorylated forms of the largest subunit of RNA pol II

phospho Ser2

RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation

phospho Ser5

RNA pol II Serine 5 phosphorylation is confined to promoter regions and is necessary for the initiation of transcription.