References for Solexa sequencing technology

Platform Comparison

• A comparison of microarray and MPSS technology platforms for expression analysis of Arabidopsis. (2007) Chen J, Agrawal V, Rattray M, West MA, St Clair DA, Michelmore RW, Coughlan SJ, Meyers BC. BMC Genomics. 8:414. PMID:17997849

• Analysis of tag-position bias in MPSS technology. (2006) Chen J, Rattray M. BMC Genomics. 7:77. PMID: 16603069

• Application of Affymetrix array and Massively Parallel Signature Sequencing for identification of genes involved in prostate cancer progression. Oudes AJ, Roach JC, Walashek LS, Eichner LJ, True LD, Vessella RL, Liu AY. (2005) BMC Cancer. 5:86. PMID:16042785

  • Comparison of Affy with MPSS (Lynx). MPSS gene expression counted anything with 1 or more tags at 3' ends of known transcripts. Tags were summed for a locus (Gene ID).

• The use of MPSS for whole-genome transcriptional analysis in Arabidopsis. (2004) Meyers BC, Tej SS, Vu TH, Haudenschild CD, Agrawal V, Edberg SB, Ghazal H, Decola S. Genome Res. 14(8):1641-53. PMID:15289482

• Analysis of the transcriptional complexity of Arabidopsis thaliana by massively parallel signature sequencing. (2004) Meyers BC, Vu TH, Tej SS, Ghazal H, Matvienko M, Agrawal V, Ning J, Haudenschild CD. Nat Biotechnol. (8):1006-11. PMID:15668391

• Statistical analysis of MPSS measurements: application to the study of

LPS-activated macrophage gene expression. (2005). Stolovitzky GA, Kundaje A, Held GA, Duggar KH, Haudenschild CD, Zhou D, Vasicek TJ, Smith KD, Aderem A, Roach JC. PNAS 102:1402-7 PMID:15247925

Massively Parallel Signature Sequencing (MPSS), a recently developed high-throughput transcription profiling technology, has the ability to profile almost every transcript in a sample without requiring prior knowledge of the sequence of the transcribed genes. As is the case with DNA microarrays, effective data analysis depends crucially on understanding how noise affects measurements. We analyze the sources of noise in MPSS and present a quantitative model describing the variability between replicate MPSS assays. We use this model to construct statistical hypotheses that test whether an observed change in gene expression in a pair-wise comparison is significant. This analysis is then extended to the determination of the significance of changes in expression levels measured over the course of a time series of measurements. We apply these analytic techniques to the study of a time series of MPSS gene expression measurements on LPS-stimulated macrophages. To evaluate our statistical significance metrics, we compare our results with published data on macrophage activation measured by using Affymetrix GeneChips.