Protocol for making random primed probes for Northern Blots. It uses Klenow fragment to extend random DNA hexamers in the presence of an alpha labelled nucleotide. Plug in the concentration of your target DNA (blue box), and adjust the dNTP label volume if needed to account for decay.
Incubate at Room Temp. 2-4 hrs Stop reaction with 1 ul 0.5 M EDTA add 1 ul tRNA 10 mg/ml 75 ul TE remove 1.5 ul for counting/calculating specific activityPurify by Gel Filtration (G50 in a Pasteur Pipet or a spin column).