Post Processing of DNA microarray slides

Rehydration/Shampoo

Set up three baths for slides:

  1. 400 ml Shampoo Soln, 65 °C
  2. 400 ml H2O
  3. 400 ml 95% Ethanol
Preheat Shampoo solution to 65 C:     Shampoo Soln 400 ml
60 ml 20x SSC
4 ml 20% SDS
336 ml distilled H2O
Succinic Anhydride Blocking

Materials:     335 ml 1-methyl-2-pyrrolidinone
5.5 g Succinic Anhydride
15 ml 1M Na Borate pH 8 (premeasure into 15 ml conical)
   
Rotating platform shaker
Empty slide dish
400 ml 95% EtOH bath
95 °C bath in beaker large enough to rotate slide rack


Chris Seidel