Cla1	AT^CGAT		NEB #4

BamH1	G^GATCC		NEB BamH1		150 mM NaCl
						10  mM Tris-HCl pH 8
						10  mM MgCl
						 1  mM DTT
						100 ug/ml BSA

Bgl II	A^GATCT		NEB #3	
										
Dra1	TTT^AAA		NEB #4

EcoR1	G^AATTC		NEB EcoR1		50  mM NaCl
						100 mM Tris-HCl pH 7.5
						10  mM MgCl

EcoRV	GAT^ATC		NEB #2

HindIII	A^AGCTT		NEB #2

Pst1	CTGCA^G		NEB #3	

Sal I	G^TCGAC		NEB #Sal I		150 mM NaCl
						10  mM Tris-HCl pH 8
						10  mM MgCl
						 1  MM DTT
						100 ug/ml BSA


SmaI	CCC^GGG		NEB #4

XmaI	C^CCGGG		NEB #1