- Common Protocols -

Procedures

Array Experiment Overview Genomic Array Protocols oligo-dT spin column mRNA purification   (pdf) NEB oligo-dT aliquots mRNA purification   (pdf) OligoTex mRNA purification   (pdf) Linear Acrylamide - Carrier for precipitations Fluorescent Probe Preparation v1.1 - amino-allyl method for RT labeling. (v1.0) Slide Hybridization (v1) Post-hyb Slide Wash Quality Control 9-mer Hybridization   (pdf) - check your array printing by quickly lighting up the spots. Post-processing of DNA microarrays - Shampoo Method (v2) | Post-processing of DNA microarrays (v1) Post-processing of DNA microarrays printed with long oligonucleotides - Shampoo Method (v2.0)   |   Post-processing of DNA microarrays printed with long oligonucleotides (v1.0)   (pdf) Post-processing of DNA microarrays with mixed targets (PCR products and 70-mers on same slide) 16 pin Array Deconvoluter Block Row Column list generator GenePix GAL file header generator Expanding a list with VLOOKUP Venn Diagram Generator Examining RNA samples with Nano Chip on Agilent Bioanalyzer. Simple Alkaline Lysis Minipreps Yeast Transformation ElectroCompetent E. coli Anderson Protein Gel - like Laemmli but runs more like a gradient gel, giving a broader range of separation per gel. Also good for separating phosphorylated proteins. Quick Silver Stain Random Hexamer Primed Probe - make hot probes easily. YGD - Simple Yeast Gene Database. Concise gene info. Openly curated. Contains graphical map of each gene blasted against all other yeast genes. GeneArrays list Archive How to make coffee. (2.1 MB QT video) How to build a Linear Servo High Speed Arrayer.

Other Information

My Library Random Tips and Scripts that help with Genomics Notes on using R Common Restriction Enzymes | Comprehensive List Codon Table Duggleby DNA Size Mapper - It's in alpha version (i.e. it's really crude at this point, but it actually works). Random Number Generator - Need to randomize some data? OD660 vs Number of Yeast Cells Yeast Nutrient Table DNA Markers Lambda cut with HindIII GibcoBRL 1 Kb Ladder (View 1) GibcoBRL 1 Kb Ladder (View 2) GibcoBRL 1 Kb Plus Ladder Protein MW Markers - BioRad Low MW   |   High MW Chris Seidel